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The vagina, unlike other systems is highly dynamic with respect to absorption of drugs, their metabolism and their elimination [ 3 ]. Evaluation of bioadhesive force and spreadability. Direct application of gels onto the infected sites of the vagina might be difficult, inconvenient as well as have frequent dosing because the conventional gels do not remain for long time at the site of application. All the formulation evaluated for Viscosity measurement was appropriate for insitu gelling system. Such phase change polymer which solidify in the vaginal cavity, might indeed have a long contact time but will probably interfere with sexual intercourse. The In-Vitro drug release was performed in sink condition, by means of Franz Diffusion cell, with water jacketed receptor chamber 20 ml and donor chamber thermo stated at 37 0 C.
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Bacterial vaginosis is caused by a change or imbalance in the types of the bacteria normally found in the vagina and causes an overgrowth of organisms. The effect of polymers with different concentration was evaluated with respect to their biological response. The pH of the mixture adjusted to 4. The spreadability of formulated gels was decreased as the concentration of polymer increased. The In-Vitro drug release was performed in sink condition, by means of Franz Diffusion cell, with water jacketed receptor chamber 20 ml and donor chamber thermo stated at 37 0 C. In addition to temperature there are other environmental triggers of gelation, such as calcium and pH. The method is based on the measurements of the tensile strength or shear stress required to break the adhesive bond between the model membrane and the test formulation.
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Invitro release behavior for developed formulation. The spreadability plays an important role in patient compliance and helps in uniform application of gel to the skin. The in situ gel forming polymeric formulations offer several advantages like sustained and prolonged action in comparison to conventional drug delivery systems. At this viscosity a dose volume of about 3. For effective vaginal delivery of antimicrobial agents, the drug delivery system should reside at the site of infection for a prolonged period of time. Physical Evaluation of the formulation:. The IR absorption spectra of the pure drug and drug-excipient mixture were taken in the range of — cm -1 using KBR disc method.
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Addition of a typical acidic buffer will probably have only a transitory effect on resident pH, whereas employing a bioadhesive polymer with an acidic pKa builds the acidifying component into the polymer, sustained the pH. The numbers of polymer those are liquid at room temperature become solid at body temperature. A good gel takes less time to spread and will have high spreadability [ 6 ]. The spreadability of formulated gels was decreased as the concentration of polymer increased. Vaginal preparations, although generally perceived as safer most, still they are associated with a number of problems, including multiple days of dosing, dripping, leakage and messiness, causing discomfort to users and expulsion due to the self-cleansing action of the vaginal tract [ 1 , 2 ]. The processed cellophane membrane was used, simulating the vaginal in vivo condition like vaginal epithelial barrier.
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